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By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)

Volume three is dedicated to the newest diagnostic know-how for virus illnesses, quite molecular methodologies.

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This process continues in a selfsustained cyclic fashion under isothermal conditions (42°C) until components in the reaction become limiting or inactivated (enzymes). , 1991); circles, reverse transcriptase; diamonds, T7 RNA polymerase; TCS, target complementary sequence. 40 D. Y. R. GINGERAS C. , 1990a; Kwoh and Kwoh, 1990; Gingeras and Kwoh, 1991; Persing, 1991). Interestingly, the 3SR amplification reaction has several features which distinguish it from other methods for nucleic acid amplification.

J. Am. Med. Assoc. 258, 1196-1199. , Vakudima, A. and LeMay, M. L. (1990). J. Pediatrics 116,372-376. , Kellogg, D. , Levenson, C. and Sninsky, J. (1990). Nucleic Acid Res. 18, 999-1005. , Mack, D. , Mullis, K. B. Poiesz, B. and Erlich, G. (1987). J. Virol. 61, 1690-1694. , Mack, D. , Erlich, G. D. Poiesz, B. J. and Dock, N. L. (1989). In HIV detection by genetic engineering methods. (P. A. Luciw and K. S. ), pp. 243-255. Marcel Dekker, New York. Larder, B. A. D. (1989). Sdence 246, 1155-1158. Larder, B.

This latter person was seroreactive by whole virus EIAS, type-specific peptide EIAS, and Western blots for both viruses. In addition PBMCS from this person contained proviral sequences of both HIV-1 and HIV-2 as determined by PCR. X. HIV INFECTION IN INFANTS HIV transmission via blood and blood product transfusions has virtually been eliminated in mothers developed countries because of donor deferral and screening of the blood supply. Thus, perinatal or vertical transmission currently accounts for nearly all incident HIV infection in prepubertal children.

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