By Luis Enjuanes
Human coronaviruses triggered the SARS epidemic that contaminated greater than 8000 humans, killing approximately ten percentage of them in 32 nations. This publication offers crucial details on those viruses and the advance of vaccines to regulate coronavirus infections.
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Extra resources for Coronavirus replication and reverse genetics
G. L. i. if infection was at 28C–30C) and then declined to about 20% of its maximum rate but interestingly did not cease. i. prevented viral RNA synthesis; addition of the drug after RNA synthesis was ongoing led in its inhibition. Unlike alphavirus but similar to picornavirus replication, both MHV plus- and minus-strand polymerase activities were unstable but in MHV they differed in their turnover rates: Minus strand synthesis was almost immediately inhibited after translation inhibition, that is, addition of cycloheximide or puromycin, whereas both genome and subgenomic mRNA syntheses continued for 30–60 min before declining slowly.
Unlike alphavirus but similar to picornavirus replication, both MHV plus- and minus-strand polymerase activities were unstable but in MHV they differed in their turnover rates: Minus strand synthesis was almost immediately inhibited after translation inhibition, that is, addition of cycloheximide or puromycin, whereas both genome and subgenomic mRNA syntheses continued for 30–60 min before declining slowly. The short half-life of the coronavirus minus-strand polymerase activity may reflect a possible role in minus strand synthesis of intermediate or partially processed polyproteins, whose further processing leads to loss of this enzymatic activity.
These together comprise a 30 coterminal nested set (Fig. 1). The mRNA species vary in size from one-third (mRNA 2) to about one-twentieth (mRNA 7) of the genome. For MHV-A59 plus strands (genomes and subgenomic mRNA) are produced in large amounts and, at about 1% of this number, minus strands of both genome- and subgenomic length that serve as the templates for genome and subgenomic mRNA synthesis (Sawicki and Sawicki 1986; Sawicki and Sawicki 1990). On infection the first open reading frame (ORF-1) in the genome of MHV-A59, which is ~20,000 nt and divided into Rep1a and Rep1b by a ribosomal frameshift, is translated as two large polyproteins, pp1a and pp1ab (Fig.